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KMID : 0613820030130020223
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Journal of Life Science
2003 Volume.13 No. 2 p.223 ~ p.229
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Antifungal Activity of Korean Radish (Raphanus sativaus L) Extracts Against Pathogenic Plant
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Hwang Chul-Wan
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Abstract
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A study of the anti-fungal properties in Korean radish was conducted using a variety of purification procedures such as Extrelut column, RP(Reverse Phase) C18 Column Chromatography, HPLC etc. to separate anti-fungal substances from Korean radish juices to test them against a common gray mold called Botrytis cenerea. Dialysis tube operation showed that these substances were presumably thermostable compounds with low molecular mass (less than 3.5 kDa). Differences of anti-fungal activities depending upon types of radishes used did not show any noticeable variation. The antifungals were presumably composed of more than 5 compounds. Among these, the most anti-fungal fraction was analyzed by HPLC in which one peak was obtained. Disease-affected plants were inoculated with 10㎎ of Extrelut fraction and results showed similar anti-fungal activity to pesticides suggesting possible usage of these substances as environmentally friendly antibiotics.
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KEYWORD
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Antifungals, Botrytis cenerea, Korean radish juices, Raphanus sativaus
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